Cell Based Assays
Invitrogen™ SP-DiOC18(3) (3,3'-Dioctadecyl-5,5'-Di(4-Sulfophenyl)Oxacarbocyanine, Sodium Salt)
When applied to cells, dye diffuses laterally within the plasma membrane
Invitrogen™ Ethidium Homodimer-1 (EthD-1)
The cell-impermeant viability indicator ethdium homodimer-1 (EthD-1) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA.
Cytometric Bead Array (CBA) Mouse/Rat Soluble Protein Master Buffer Kit, 100 tests, BD
Employs particles with discrete fluorescence intensities to detect soluble analytes at very low concentrations
Cytometric Bead Array Wash Buffer, BD
To be used with the BD CBA Flex Set assays
Invitrogen™ FluoZin™-3, AM, cell permeant
Zn2+-selective indicator with a structure that resembles fluo-4
BD Violet Proliferation Dye 450
For monitoring of cell divisions completed by single cells, intracellular staining
BD Apoptosis Detection Kit (APO-Direct)
Single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.
MilliporeSigma™ Chemicon™ MTT Reagent A
For study of cell and mitochondrial health
Invitrogen™ CoroNa™ Green, cell impermeant
A sodium ion indicator that exhibits an increase in green fluorescence emission intensity upon binding Na+, with little shift in wavelength
R&D Systems™ Poly(ADP-ribose) Polymer/pADPr
Synthesized using poly(ADP-ribose) polymerase (PARP-1) in the presence of NAD, cleaved from PARP-1, and purified by gel filtration
MilliporeSigma™ Acetyl-Histone H3 Immunoprecipitation (ChIP) Assay Kit
Used to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-Acetyl-Histone H3
MilliporeSigma™ Acetyl-Histone H4 Immunoprecipitation (ChIP) Assay Kit
Used to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-Acetyl-Histone H4, ChIP grade rabbit antiserum
MilliporeSigma™ PI3 Kinase Activity/Inhibitor ELISA
Used to measure & quantify PI 3-Kinase levels
Agilent Technologies™ XF Hu T Cell Activation Assay Kit
XF Hu T Cell Activation Assay Kit contains one vial of 2 mL ImmunoCult human CD3/CD28 T cell activator and two vials of XF 2-deoxy-D-glucose. For use with Seahorse XF HS Mini, XFp and XFe/XF96 analyzers.